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The gene function encoding translation elongation factor eEF3 is partially conserved across fungi.
Front Microbiol
August 2024
Laboratory of mRNA and Cancer, Unit of Biomedical Research on Cancer, National Institute of Cancer (Instituto Nacional de Cancerología, INCan), Mexico City, Mexico.
Introduction: Translation is a fundamental process of life. In eukaryotes, the elongation step of translation is highly conserved and is driven by eukaryotic translation elongation factors (eEF)1A and eEF2. A significant variation of the elongation is the activity of eukaryotic elongation factor (eEF) 3 in encoded by the gene yeast elongation factor () with orthologs in all fungal species, a few algae, and some protists.
View Article and Find Full Text PDFUtilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered Corynebacterium glutamicum.
Enzyme Microb Technol
January 2024
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan. Electronic address:
Itaconic acid is a promising biochemical building block that can be used in polymer synthesis. Itaconic acid is currently produced in industry by the natural producer fungus Aspergillus terreus using glucose as a main carbon source. Most research for itaconic acid production using lignocellulosic-based carbon sources was carried out by A.
View Article and Find Full Text PDFEffect of different metabolic pathways on itaconic acid production in engineered Corynebacterium glutamicum.
J Biosci Bioeng
August 2023
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan. Electronic address:
Article Synopsis
- Itaconic acid (IA) is a promising bio-based material for the polymer industry, with production typically relying on the cis-pathway from the cadA gene in Aspergillus terreus.
- This study explores the production of IA using engineered strains of Corynebacterium glutamicum expressing two alternative pathways: the cis-pathway (Irg1 from Mus musculus) and the trans-pathway (Adi1 and Tad1 from Ustilago maydis).
- Results indicate that the trans-pathway significantly outperforms the cis-pathway, achieving higher IA production yields (up to 12.25 g/L) when using different carbon sources such as glucose, maltose, and sucrose.
Techno-Economic Analysis as a Driver for Optimisation of Cellobiose Lipid Fermentation and Purification.
Front Bioeng Biotechnol
June 2022
Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Stuttgart, Germany.
Cellobiose lipids (CL) are glycolipids synthesized by species with potential application as detergents or in cosmetics. This study identified process optimisation potential for CL fermentation based on process modelling and techno-economic analysis. Using a stoichiometric equation based on laboratory data, we calculated the maximum possible CL yield Y of 0.
View Article and Find Full Text PDFFactors Affecting the Synthesis of Cellobiose Lipids by .
Front Bioeng Biotechnol
November 2020
Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Stuttgart, Germany.
Cellobiose lipids (CL) are extracellular glycolipids that are produced by many microorganisms from the family . The sugarcane smut fungus has been long known as a producer of the glycolipids mannosylerythritol lipids (MEL) and was recently described to additionally secrete CL as a byproduct. In fact, we identified 11 homologous genes in by analysis sharing a high similarity to the CL biosynthesis gene cluster of .
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