sp. nov., isolated from citrus rhizosphere.

Int J Syst Evol Microbiol

Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou City, Fujian Province, 350002, PR China.

Published: May 2023

Three Gram-positive-staining strains FJAT-49754, FJAT-49682 and FJAT-49731 were isolated from the citrus rhizosphere soil sample. These strains showed the highest 16S rRNA gene sequence similarity with the type strain of (97.8-97.9 %). The 16S rRNA gene sequence similarities between strains FJAT-49754, FJAT-49682, and FJAT-49731 were 99.9 %. The average nucleotide identity (ANI) values between strains FJAT-49754, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus were below 95 %, which were below the cut-off level for prokaryotic species delineation. The above results suggest that strains FJAT-49754, FJAT-49682 and FJAT-49731 belong to a novel species of the genus . Growth of strain FJAT-49754 was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %). MK-7 was the only menaquinone detected in strain FJAT-49754, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids of strain FJAT-49754 were anteiso-C iso-C and C. The genomic DNA G+C content of strain FJAT-49754 was 38.7 %. Based on the above results, strain FJAT-49754 represents a novel species of the genus , for which the name sp. nov., is proposed. The type strain is FJAT-49754 (=CCTCC AB 2019211=LMG 31589).

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http://dx.doi.org/10.1099/ijsem.0.005833DOI Listing

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