The hydrogen peroxide (HO) measurement is considered highly important in industrial wastewater quality assessment, environmental protection, and disease detection. Here, a simple high-performance paper-based sensor is proposed for rapid and in situ detection of HO. To this end, 3,3',5,5'-tetramethylbenzidine is embedded in the sensor to act as a color indicator, whose reaction with hydrogen peroxide is catalyzed by a silver nanozyme modified by sericin. The result of the reaction clarified by the appearance of blue color in the sensor detection zone is received by a portable scanner, while also calculating its intensity by image analysis software. This method is sensitive to hydrogen peroxide in the concentration range of 0.5‒240 mg/dL, providing a detection limit of 0.15 mg/dL. The ability of the sensor to determine glucose is also evaluated by adding a layer containing glucose oxidase enzyme to the sensor structure. A desirable response is obtained in the range of 1.0‒160 mg/dL, together with a detection limit of 0.37 mg/dL. Accordingly, the proposed sensor shows satisfactory results compared to clinical methods for monitoring the amount of glucose in biological samples such as serum and saliva.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10151347 | PMC |
http://dx.doi.org/10.1038/s41598-023-34299-1 | DOI Listing |
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