A simple method for the automatic inoculation of broth culture tubes is described. An incubator is plugged into an electrical outlet timer. Heating of the incubator causes wax-like coconut oil to melt and allow an inoculum-coated bead to drop into sterile liquid medium.
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http://dx.doi.org/10.1016/j.mimet.2023.106727 | DOI Listing |
Microb Pathog
December 2024
Lanzhou Shouyan Biotechnology Co., Ltd, Lanzhou, 730046, China; State Key Laboratory for Animal Disease Control and Prevention, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. Electronic address:
Foot-and-mouth disease virus (FMDV) causes significant financial losses in animal farms worldwide. Specific antibody monitoring of FMDV post-vaccination is important for evaluating vaccine efficacy, as well as the prevention and control of foot-and-mouth disease (FMD). In this study, we developed a fully automatic tubular chemiluminescence immunoassay method based on magnetic particles (A-MPCLIA) to specifically detect antibodies against FMDV serotype A.
View Article and Find Full Text PDFJ Med Internet Res
November 2024
Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, United States.
Background: Adverse events (AEs) associated with vaccination have traditionally been evaluated by epidemiological studies. More recently, they have gained attention due to the emergency use authorization of several COVID-19 vaccines. As part of its responsibility to conduct postmarket surveillance, the US Food and Drug Administration continues to monitor several AEs of interest to ensure the safety of vaccines, including those for COVID-19.
View Article and Find Full Text PDFBMC Med
November 2024
Fondazione Policlinico Universitario A. Gemelli IRCCS, Università Cattolica del Sacro Cuore, Rome, Italy.
Background: Long-COVID symptoms remain incompletely defined due to a large heterogeneity in the populations studied, case definitions, and settings of care. The aim of this study was to assess, in patients accessing care for Long-COVID, the profile of symptoms reported, the possible clustering of symptoms and cases, the functional status compared to pre-infection, and the impact on working activity.
Methods: Multicentre cohort study with a collection of both retrospective and prospective data.
Vet Sci
October 2024
Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.
Citric acid by-products in animal feed pose a sustainability challenge. species are commonly used for fermenting and improving the nutritional quality of feedstuffs or by-products. An experiment was conducted to enhance the nutritional value of citric acid by-products through fermentation with I9 for animal feed.
View Article and Find Full Text PDFPublic Health Res Pract
October 2024
National Centre for Immunisation Research and Surveillance, Sydney, NSW, Australia; School of Public Health, Faculty of Medicine and Health, University of Sydney, NSW, Australia.
Unlabelled: Objectives and importance of the study: Pharmacist-administered vaccination has expanded in Australia but has not been comprehensively assessed. We aimed to assess the pharmacists' role in vaccination in Australia before and during the first year of the COVID-19 pandemic, as well as the completeness of data on pharmacist-administered immunisations.
Study Type: Cross-sectional study.
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