A healthy 49-year-old female developed intractable watery diarrhea after the Pfizer SARS-Cov2 mRNA vaccination. She was subsequently diagnosed with collagenous colitis (CC). She had no prior history of medication use, suggesting of vaccination being the trigger. CC or lymphocytic colitis should be considered as differential diagnoses for persistent watery diarrhea after SARS-Cov2 mRNA vaccination.
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http://dx.doi.org/10.1002/jgh3.12885 | DOI Listing |
Physiology (Bethesda)
March 2025
Department of Critical Care Medicine, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China.
In recent years, the introduction of mRNA vaccines for SARS-CoV2 and RSV has highlighted the success of the mRNA technology platform. Designing mRNA sequences involves multiple components and requires balancing several parameters, including enhancing transcriptional efficiency, boosting antigenicity, and minimizing immunogenicity. Moreover, changes in the composition and properties of delivery vehicles can also affect vaccine performance.
View Article and Find Full Text PDFMethods Protoc
January 2025
Vavilov Institute of General Genetics, Russian Academy of Sciences, 119991 Moscow, Russia.
Reverse transcription polymerase chain reaction (RT-PCR) is an important tool for the detection of target RNA molecules and the assay of RNA pathogens. Coupled RT-PCR is performed with an enzyme mixture containing a reverse transcriptase and a thermostable DNA polymerase. To date, several biotechnological companies offer artificial thermostable DNA polymerases with a built-in reverse transcriptase activity for use in the coupled RT-PCR instead of the enzyme mixtures.
View Article and Find Full Text PDFACS Bio Med Chem Au
February 2025
Research Center for Medical Sciences, the Jikei University School of Medicine, 163-1 Kashiwa-shita, Chiba 277-0004, Japan.
Poly(ethylene glycol)-related immune responses have been a great concern regarding mRNA vaccination for the SARS-CoV2 virus, because PEG-lipids are an essential component for the lipid nanoparticles of mRNA vaccines. Meanwhile, no research has elucidated the mechanisms underlying hapten-like PEG-related immunogenicity. For the current study, we uncovered a process by which haptenic PEGs transition into immunogenic PEG-conjugates by means of ELISA and microfluidic diffusional sizing (MDS).
View Article and Find Full Text PDFHeliyon
January 2025
Pediatric Infectious Diseases Unit, Department of Pediatrics, Gregorio Marañón University Hospital, Madrid, Spain.
Objective: The aim of this prospective cohort study is to analyse the humoral and cellular vaccine responses in paediatric heart transplant recipients (HTR, n = 12), and compare it with the response in healthy controls (HC, n = 14). All participants were 5-18 years old and vaccinated with mRNA vaccine against SARS-CoV-2 between December 2021 and May 2022.
Methods: The humoral response was measured by quantifying antibody titers against SARS-CoV-2 spike protein (anti-S).
Pediatr Res
January 2025
Clinical Immunology and Vaccinology Unit, Bambino Gesù Children's Hospital IRCCS, Rome, Italy.
Background: Recent proteomic studies have documented that Long COVID in adults is characterized by a pro-inflammatory signature with thromboinflammation. However, if similar events happen also in children with Long COVID has never been investigated.
Methods: We performed an extensive protein analysis of blood plasma from pediatric patients younger than 19 years of age Long COVID and a control group of children with acute COVID-19, MIS-C, and healthy controls resulted similar for sex distribution and age.
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