Background And Objectives: species are known to be one of the common causes of keratitis. This study was conducted to identify spp. causing keratitis and to investigate their genetic diversity using and gene sequences.
Materials And Methods: Twenty-four clinical isolates of were isolated from the patient with keratitis. Phylogenetic analysis of two-locus of the 24 clinical isolates and three reference strains was carried out using the maximum parsimony and RAxML methods.
Results: Based on gene sequences of the 24 clinical isolates, 17, 4, and 3 isolates were identified as species complex (FSSC), species complex (FFSC), and , respectively. FFSC include (n=1), (n=1), (n=1), and (n=1), respectively.
Conclusion: Given that sequence of a sole gene can be challenging and on the other hand, due to the high resistance to antifungal drugs, identification of species is of substantial significance. In this study, by designing a novel set of primers for the area and using primer, we were able to differentiate 24 spp. isolated from patients with keratitis.
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| http://dx.doi.org/10.18502/ijm.v14i3.9753 | DOI Listing |
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