The rise of antimicrobial-resistant bacterial infections is a crucial health concern in the 21st century. In particular, antibiotic-resistant causes difficult-to-treat infections associated with high morbidity and mortality. Unfortunately, the number of effective therapeutic interventions against antimicrobial-resistant infections continues to decline. Therefore, discovery and development of alternative treatments are necessary. Here, we present pre-clinical efficacy studies on an anti- therapeutic monoclonal antibody. Using hybridoma technology, we generated a monoclonal antibody and characterized its binding to using ELISA and fluorescence correlation spectroscopy. We also characterized its function and against . The anti- antibody (WVDC-5244) bound clinical strains of various serotypes , even in the presence of alginate exopolysaccharide. In addition, WVDC-5244 induced opsonophagocytic killing of in J774.1 murine macrophage, and complement-mediated killing. In a mouse model of acute pneumonia, prophylactic administration of WVDC-5244 resulted in an improvement of clinical disease manifestations and reduction of burden in the respiratory tract compared to the control groups. This study provides promising pre-clinical efficacy data on a new monoclonal antibody with therapeutic potential for infections.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10140502 | PMC |
| http://dx.doi.org/10.3389/fcimb.2023.1117844 | DOI Listing |
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