Genetic Clarification of Strains Bred and Cultivated in Korea Using the ITS and IGS1 rDNA Region Sequences.

Mycobiology

Department of Microbiology, Division of Biological Sciences, Dankook University, Cheonan, Republic of Korea.

Published: March 2023

is one of the broadly cultivated edible mushrooms in Korea. Most of the Korean strains used for cultivation and breeding are known as . Recently, this species has been reported to belong to a species complex. Therefore, this study was carried out to genetically clarify the bred and cultivated Korean strains. The internal transcribed spacer (ITS) and IGS1 rDNA region sequences were determined from 10 strains by PCR and sequencing. Variation in the nucleotide sequence and sequence length of the two rDNA regions were found among the seven strains. A maximum-likelihood (ML) phylogenetic tree based on the ITS sequences clearly placed all the 10 Korean strains in the clade of the complex. is diverged from . An ML phylogenetic tree based on the IGS1 sequences revealed the close relationship between Korean strains to Chinese strains. But each strain could be distinguishable by the IGS1 sequence. Furthermore, progeny strains in the seven Korean strains could be differentiated from their parental strains by the IGS1 sequence based phylogenetic tree. Our results are expected to be used to complement the distinction of domestic cultivars.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142399PMC
http://dx.doi.org/10.1080/12298093.2023.2182024DOI Listing

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