AI Article Synopsis
- Single-cell RNA sequencing (scRNA-seq) is a powerful tool for analyzing specific immune cell populations involved in inflammatory diseases, but isolating viable cells from human skin can be difficult.
- This text outlines a protocol that details the steps for obtaining skin biopsy samples, breaking them down with enzymes, and using flow cytometry to isolate immune cells.
- It also introduces computational methods for analyzing the sequencing data, with references to more comprehensive guidelines in Cook et al. (2022) and Liu et al. (2022).
Article Abstract
Single-cell RNA sequencing (scRNA-seq) allows for high-resolution analysis of transcriptionally dysregulated cell subpopulations in inflammatory diseases. However, it can be challenging to properly isolate viable immune cells from human skin for scRNA-seq due to its barrier properties. Here, we present a protocol to isolate high-viability human cutaneous immune cells. We describe steps for obtaining and enzymatically dissociating a skin biopsy specimen and isolating immune cells using flow cytometry. We then provide an overview of downstream computational techniques to analyze sequencing data. For complete details on the use and execution of this protocol, please refer to Cook et al. (2022) and Liu et al. (2022)..
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10173011 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2023.102239 | DOI Listing |
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