AI Article Synopsis
- - Plumula Nelumbinis, the seed embryo of the lotus plant (Nelumbo nucifera), is popular in East Asia for making tea and supplements, and researchers isolated new and known alkaloids from it.
- - Six new bisbenzylisoquinoline alkaloids and seven known alkaloids were identified, with specific compounds demonstrating over 50% inhibition of MOVAS cell migration, outperforming the positive control, cinnamaldehyde.
- - Further studies indicated that nelumboferine, one of the active alkaloids, inhibits cell migration and proliferation by affecting the ORAI2/Akt signaling pathway.
Article Abstract
Plumula Nelumbinis, the embryo of the seed of Nelumbo nucifera Gaertn, is commonly used to make tea and nutritional supplements in East Asian countries. A bioassay-guided isolation of Plumula Nelumbinis afforded six previously undescribed bisbenzylisoquinoline alkaloids, as well as seven known alkaloids. Their structures were elucidated by extensive analysis of HRESIMS, NMR, and CD data. Pycnarrhine, neferine-2α,2'β-N,N-dioxides, neferine, linsinine, isolinsinine, and nelumboferine, at 2 μM significantly suppressed the migration of MOVAS cells with inhibition ratio above 50%, more active than that of the positive control cinnamaldehyde (inhibition ratio 26.9 ± 4.92%). Additionally, neferine, linsinine, isolinsinine, and nelumboferine, were also active against the proliferation of MOVAS cells with inhibition ratio greater than 45%. The preliminary structure-activity relationships were discussed. Mechanism studies revealed that nelumboferine inhibited the migration and proliferation of MOVAS cells by regulating ORAI2/Akt signaling pathway.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.phytochem.2023.113700 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fermented Against Atopic Dermatitis Through AKT/mTOR and Jun Pathways.
Pharmaceuticals (Basel)
December 2024
Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China.
Atopic dermatitis (AD) is a chronic inflammatory skin disorder that has attracted global attention, and alkaloids from have been shown to have anti-inflammatory activity. Fermentation has been used for the structural modification of natural compounds to improve bioavailability and activity, but the AD therapeutic efficacy and mechanism of the fermented (FPN) are still unclear. The potential targets of FPN for AD were preliminarily screened using network pharmacology, and then PCR and WB were used to prove the therapeutic effect of FPN in AD.
View Article and Find Full Text PDFBenzylisoquinoline alkaloids from the embryo of Nelumbo nucifera and their antioxidant effects.
Fitoterapia
January 2025
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing Key Laboratory of Polymorphic Drugs, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, China. Electronic address:
Seven benzylisoquinoline alkaloids, including three new (1-3) and four known compounds (4-7), were isolated from the embryo of Nelumbo nucifera (Plumula Nelumbinis). Their structures were elucidated by UV, IR, HRESIMS, 1D and 2D NMR, and ECD spectra, as well as optical rotations. All compounds were tested for the antioxidant activity.
View Article and Find Full Text PDFDetermination of doping higenamine in Chinese herbal medicines and their concentrated preparations by LC-MS/MS.
J Pharm Biomed Anal
August 2024
Department of Sports Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung City 80708, Taiwan; Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung City 80708, Taiwan. Electronic address:
The World Anti-Doping Agency (WADA) has included higenamine in the β2 agonist (S3) category of the Prohibited List since 2017 due to its pharmacological effects on adrenergic receptors. Although higenamine contained in Chinese herbal medicines has been identified by previous studies, comprehensive investigation on the higenamine content of Chinese herbs and their concentrated preparations is still required. This study aimed to determine the levels of higenamine in Chinese medicinal materials and their concentrated preparations used in Chinese medicine prescriptions in Taiwan.
View Article and Find Full Text PDFDevelopment of CO-switchable deep eutectic solvent-based liquid-liquid microextraction approach: Application in urinary excretion and tissue distribution studies.
Anal Chim Acta
June 2024
Hebei Key Laboratory of Organic Functional Molecules, College of Chemistry and Materials Science, Hebei Normal University, Shijiazhuang, Hebei, 050024, China. Electronic address:
Background: Pharmacokinetic studies are pivotal in drug development, focusing on absorption, distribution, and excretion of active compounds. Effective sample preparation methods play a crucial role in these studies. Traditional techniques like protein precipitation and liquid-liquid extraction often involve toxic solvents and are time-consuming.
View Article and Find Full Text PDFLiensinine alleviates sepsis-induced acute liver injury by inhibiting the NF-κB and MAPK pathways in an Nrf2-dependent manner.
Chem Biol Interact
June 2024
Department of Vascular Surgery, The First People's Hospital of Lianyungang, Lianyungang, 222005, China. Electronic address:
Sepsis remains a serious public health issue that needs to be addressed globally. Severe liver injury caused by sepsis increases the risk of death in patients with sepsis. Liensinine (Lie) is one of the primary active components in Plumula nelumbinis and has anti-inflammatory and antioxidant effects.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!