AI Article Synopsis
- The study explores the potential of using green and biodegradable deep eutectic solvents (DES) as nonaqueous solvents to improve enzyme biosensor performance.
- The research specifically focuses on monitoring the activity of the tyrosinase enzyme in a DES made from choline chloride and glycerol, with phenol as the target analyte.
- By utilizing a gold-nanoparticle-modified electrode, the study paves the way for the development of eco-friendly electrochemical biosensors suitable for analyzing phenols in various environments, including gas phase.
Article Abstract
The use of green, inexpensive, and biodegradable deep eutectic solvents as nonaqueous solvents and electrolytes could be a useful way to potentially improve the enzyme biosensor performance as well as a profitable strategy to extend their use in the gas phase. However, enzyme activity in these media, although fundamental for their implementation in electrochemical analysis, is still almost unexplored. In this study, an electrochemical approach was employed to monitor tyrosinase enzyme activity in a deep eutectic solvent. This study was performed in a DES consisting of choline chloride (ChCl) as a hydrogen bond acceptor (HBA) and glycerol as a hydrogen bond donor (HBD), while phenol was chosen as the prototype analyte. The tyrosinase enzyme was immobilized on a gold-nanoparticle-modified screen-printed carbon electrode, and its activity was monitored following the reduction current of orthoquinone produced by the tyrosinase biocatalysis of phenol. This work represents a first step toward the realization of green electrochemical biosensors capable of operating in both nonaqueous and gaseous media for the chemical analysis of phenols.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10143261 | PMC |
| http://dx.doi.org/10.3390/s23083915 | DOI Listing |
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