Nanofiber scaffolds of polyvinyl alcohol, silk fibroin from cocoons, and silver nanoparticles were developed as a substrate for MG-63 growth. The fiber morphology, mechanical properties, thermal degradation, chemical composition, and water contact angle were investigated. In vitro tests were performed by the cell viability MTS test of MG-63 cells on electrospun PVA scaffolds, mineralization was analyzed by alizarin red, and the alkaline phosphatase (ALP) assay was evaluated. At higher PVA concentrations, Young's modulus (E) increased. The addition of fibroin and silver nanoparticles improved the thermal stability of PVA scaffolds. FTIR spectra indicated characteristic absorption peaks related to the chemical structures of PVA, fibroin, and Ag-NPs, demonstrating good interactions between them. The contact angle of the PVA scaffolds decreased with the incorporation of fibroin and showed hydrophilic characteristics. In all concentrations, MG-63 cells on PVA/fibroin/Ag-NPs scaffolds had higher cell viability than PVA pristine. On day ten of culture, PVA18/SF/Ag-NPs showed the highest mineralization, observed by the alizarin red test. PVA10/SF/Ag-NPs presented the highest alkaline phosphatase activity after an incubation time of 37 h. The achievements indicate the potential of the nanofibers of PVA18/SF/Ag-NPs as a possible substitute for bone tissue engineering (BTE).
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10144439 | PMC |
| http://dx.doi.org/10.3390/polym15081838 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Histology Assessment of Chitosan-Polyvinyl Alcohol Scaffolds Incorporated with CaO Nanoparticles.
Molecules
January 2025
Grupo Biomateriales Dentales, Escuela de Odontología, Universidad del Valle, Calle 4B # 36-00, Cali 760001, Colombia.
Scaffolds for regenerative therapy can be made from natural or synthetic polymers, each offering distinct benefits. Natural biopolymers like chitosan (CS) are biocompatible and biodegradable, supporting cell interactions, but lack mechanical strength. Synthetic polymers like polyvinyl alcohol (PVA) provide superior mechanical strength and cost efficiency but are not biodegradable or supportive of cell adhesion.
View Article and Find Full Text PDFEvaluation of Sericin/Polyvinyl Alcohol Mixtures for Developing Porous and Stable Structures.
Biomimetics (Basel)
January 2025
Agroindustrial Research Group, Department of Chemical Engineering, Universidad Pontificia Bolivariana, Cq. 1 #70-01, Medellín 050031, Colombia.
Fibrous by-products, including defective or double cocoons, are obtained during silk processing. These cocoons primarily contain fibroin and sericin (SS) proteins along with minor amounts of wax and mineral salts. In conventional textile processes, SS is removed in the production of smooth, lustrous silk threads, and is typically discarded.
View Article and Find Full Text PDFFabrication and characterization of in situ gelling oxidized carboxymethyl cellulose/gelatin nanofibers for wound healing applications.
Int J Biol Macromol
January 2025
Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
Although tissue engineering science has made great progress, wound healing has remained a significant clinical challenge, especially in cases of severe injuries requiring advanced treatment strategies. This study aimed to develop patient-friendly in situ gelling nanofibers composed of oxidized carboxymethyl cellulose (OCMC) and gelatin for wound healing applications. A two-axial electrospinning technique was employed to fabricate OCMC/PVA-Gelatin hybrid nanofibers.
View Article and Find Full Text PDFDesigning sustained release from nanofiber patch for paclitaxel as prospective localized nanotherapeutic delivery in breast cancer.
Int J Pharm
January 2025
Nanostructure and Biomimetic Lab, Department of Nanotechnology, University of Kashmir, Hazratbal, Srinagar 190006, Jammu and Kashmir, India. Electronic address:
The second most prevalent cause of mortality among women is breast cancer, and paclitaxel (PTX) is an effective drug for its treatment. The present work aims to develop patch-based poly(ε-caprolactone) (PCL) nanofibers incorporating PTX as a localized and sustained drug delivery system. The co-deposition of poly(vinyl alcohol) (PVA) fibers during electrospinning was allowed to improve water absorption by the scaffold, which in turn facilitated the release of drug molecules.
View Article and Find Full Text PDFFabrication and characterization of silver nanowire-coated porous alginate wet-laid webs for wound dressing applications.
Int J Biol Macromol
January 2025
Faculty of Textile Technologies and Design, Istanbul Technical University, Istanbul, Turkey. Electronic address:
Wound care presents an imposed financial burden for healthcare organizations, prompting the need for novel and cost-efficient dressings. In this study, we address this challenge by introducing a novel approach to fabricate antibacterial alginate-based fibrous materials using a combination of wet spinning and the wet-laying method, which offer advantages including structural and functional properties such as breathability, nontoxicity, biocompatibility, and cost-effectiveness. The wet spinning method was employed to develop porous and non-porous Ca-alginate fibers with diameters of 100 ± 4.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!