Rapid identification of beta-lactamase-producing strains of plays key role in diagnostics in clinical microbiology. Therefore, the aim of this study was the rapid determination of beta-lactamase's presence in isolates via indirect detection of degradation ampicillin products using MALDI-TOF MS. isolates were subjected to antibiotic resistance testing using disk diffusion and MIC methodologies. Beta-lactamase activity was tested using MALDI-TOF MS, and results were compared to spectral analysis of alkaline hydrolysis. Resistant and susceptible strains of were distinguished, and strains with a high MIC level were identified as beta-lactamase-producing. Results indicate that MALDI-TOF mass spectrometry is also suitable for the rapid identification of beta-lactamase-producing . This observation and confirmation can accelerate identification of beta-lactamase strains of in clinical microbiology, which can have an impact on health in general.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10142446 | PMC |
| http://dx.doi.org/10.3390/microorganisms11041018 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative study of labour analgesia onset with injection of loading dose through epidural needle versus catheter: A prospective, double-blinded, randomised clinical trial.
Eur J Anaesthesiol
February 2025
From the Department of Anesthesia, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, China (XC, YT, QY, LS, HL, LW, CJ, XC).
Background: Rapid onset of epidural analgesia is an important concern for the parturient. Commonly, the local anaesthetic mixture is administered through the epidural catheter. Drugs administered through the epidural needle might decrease the onset time and enhance the spread of medication within the epidural space.
View Article and Find Full Text PDFIsolation and Antibiotic Resistance Rates of complex in Mycolor TK System.
Infect Dis Clin Microbiol
December 2024
Department of Medical Pharmacology, Sivas Cumhuriyet University Faculty of Medicine, Sivas, Türkiye.
Objective: Tuberculosis (TB) is a public health problem. This study aimed to determine the growth rates and drug susceptibility levels of patients with complex (MTC) growth in cultures obtained and to compare the results with the growth rates and drug susceptibility levels found in our country and other countries. It also aimed to evaluate the results of supplementing classical methods such as Lowenstein-Jensen (LJ) with liquid TK MEDIUM and to determine the relationship between the growth rates obtained with both methods.
View Article and Find Full Text PDFMolecular identification of yeast communities isolated from nail specimens by PCR-RFLP and PCR-FSP methods.
Curr Med Mycol
May 2024
Department of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Background And Purpose: Onychomycosis is a common fungal infection that affects the nails, caused by various fungal agents. Moreover, yeast onychomycosis has increased in recent years. Yeast isolates might not be identified at the species level by conventional methods, whereas molecular methods can identify yeast isolates more accurately.
View Article and Find Full Text PDFas an emerging rare cause of septicemia among neonates and children in Western Uttar Pradesh: A case series.
Curr Med Mycol
May 2024
Department of Microbiology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow, India.
Background And Purpose: is a recently emerging nosocomial fungal pathogen. Candidemia is the fourth most prevalent cause of bloodstream Infections with mortality rates varying from 5-71%.
Materials And Methods: This was a retrospective study conducted at Uttar Pradesh University of Medical Sciences, Etawah, India, from September 2023 to February 2024.
Genome-wide identification, evolution and expression analysis unveil the role of genes in nitrogen utilization and nitrogen allocation.
Physiol Mol Biol Plants
December 2024
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010 Sichuan Province China.
Unlabelled: The rapid growth of Bamboo made the uptake and allocation of nitrogen much important. Nitrate is the main form that plant utilized nitrogen by nitrate transporters (NRTs) as well as ammonium salt. In this study, we identified 155 genes which mapped to 32 chromosomes out of 35 chromosomes in .
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!