The identification of tissue-specific promoters for gene therapeutic constructs is one of the aims of complex tumor therapy. The genes encoding the fibroblast activation protein () and the connective tissue growth factor () can function in tumor-associated stromal cells but are practically inactive in normal adult cells. Accordingly, the promoters of these genes can be used to develop vectors targeted to the tumor microenvironment. However, the efficiency of these promoters within genetic constructs remains underexplored, particularly, at the organism level. Here, we used the model of embryos to study the efficiency of transient expression of marker genes under the control of promoters of the , , and immediate early genes of (CMV). Within 96 h after the injection of vectors, the and CMV promoters provided similar equal efficiency of reporter protein accumulation. In the case of the promoter, a high level of reporter protein accumulation was observed only in certain zebrafish individuals that were considered developmentally abnormal. Disturbed embryogenesis was the factor of changes in the exogenous promoter function. The data obtained make a significant contribution to understanding the function of the human and promoters within vectors to assess their potential in gene therapy.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138699 | PMC |
| http://dx.doi.org/10.3390/ijms24087192 | DOI Listing |
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