Genome-Wide Identification, Characterization, and Expression of Genes Family in Orchardgrass.

Plant-specific TCP transcription factors regulate several plant growth and development processes. Nevertheless, little information is available about the TCP family in orchardgrass ( L.). This study identified 22 transcription factors in orchardgrass and determined their structure, phylogeny, and expression in different tissues and developmental stages. The phylogenetic tree classified the gene family into two main subfamilies, including class I and II supported by the exon-intron structure and conserved motifs. The promoter regions contained various cis-elements associated with hormones, growth and development, and stress responses, including MBS (drought inducibility), circadian (circadian rhythms), and TCA-element (salicylic acid responsiveness). Moreover, possibly regulates tillering and flowering time. Additionally, several stress treatments upregulated and , indicting their potential effects regarding regulating responses to the respective stress. This research offers a valuable basis for further studies of the gene family in other Gramineae and reveals new ideas for increasing gene utilization.