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A Novel Two-Dimensional Liquid Chromatography Combined with Ultraviolet Detection Method for Quantitative Determination of Pyridoxal 5'-Phosphate, 4-Pyridoxine Acid and Pyridoxal in Animal Plasma. | LitMetric

AI Article Synopsis

  • Vitamin B, especially vitamin B6, is crucial for maintaining overall health, and tracking its levels can reveal important connections between its content and various diseases.
  • The study developed a new method using two-dimensional liquid chromatography with a UV detector to simultaneously detect three specific forms of vitamin B6 compounds (PLP, PA, and PL).
  • The method demonstrated high accuracy and sensitivity, making it useful for research in pharmacology, pharmaceuticals, and clinical settings, with excellent resolution and detection limits for the compounds involved.

Article Abstract

Vitamin B is an indispensable micronutrient in organisms and is widely distributed in blood, tissues, and organs. Changes in the content and ratio of vitamin B6 can affect the entire physiological condition of the body, so it becomes particularly important to reveal the relationship between changes in its content and disease by monitoring vitamin B6 levels in the organism. In this study, a two-dimensional liquid chromatography-UV detector (2D-LC-UV) was used to establish a method for the simultaneous detection of PLP, PA, and PL for the first time. First, PLP, PA, and PL were extracted with plasma: 0.6 M TCA: ultrapure water = 1:2:3 (//) and then derivatized. Enrichment and preliminary separation were performed on a one-dimensional column and automatically entered into a two-dimensional column for further separation. This method exhibited good selectivity, and the correlation coefficients for the analyte calibration curves were >0.99. The detection limits for PLP, PA, and PL were 0.1, 0.2, and 4 nmol/L, respectively. The results showed that the system has high loading capacity, excellent resolution, and a good peak shape. This method is expected to provide applicability for the determination of PLP, PA, and PL in pharmacological, pharmaceutical, and clinical research.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135266PMC
http://dx.doi.org/10.3390/ani13081333DOI Listing

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