AI Article Synopsis
- The human fallopian tube epithelium (hFTE) is crucial for fertilization and early embryo development, but its small extracellular vesicles (sEVs) remain largely understudied due to previous research limitations.
- A new microfluidic platform has been developed that allows for efficient hFTE culture and collection of sEVs, revealing 295 specific proteins linked to processes like exocytosis and fertilization for the first time.
- This study connects the profiles of sEV proteins to hFTE cell-type-specific transcripts, highlighting key proteins in secretory cells that may play a role in the development of high-grade serous ovarian cancers.
Article Abstract
The human fallopian tube epithelium (hFTE) is the site of fertilization, early embryo development, and the origin of most high-grade serous ovarian cancers (HGSOCs). Little is known about the content and functions of hFTE-derived small extracellular vesicles (sEVs) due to the limitations of biomaterials and proper culture methods. We have established a microfluidic platform to culture hFTE for EV collection with adequate yield for mass spectrometry-based proteomic profiling, and reported 295 common hFTE sEV proteins for the first time. These proteins are associated with exocytosis, neutrophil degranulation, and wound healing, and some are crucial for fertilization processes. In addition, by correlating sEV protein profiles with hFTE tissue transcripts characterized using GeoMx Cancer Transcriptome Atlas, spatial transcriptomics analysis revealed cell-type-specific transcripts of hFTE that encode sEVs proteins, among which, FLNA, TUBB, JUP, and FLNC were differentially expressed in secretory cells, the precursor cells for HGSOC. Our study provides insights into the establishment of the baseline proteomic profile of sEVs derived from hFTE tissue, and its correlation with hFTE lineage-specific transcripts, which can be used to evaluate whether the fallopian tube shifts its sEV cargo during ovarian cancer carcinogenesis and the role of sEV proteins in fallopian tube reproductive functions.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135590 | PMC |
| http://dx.doi.org/10.3390/bioengineering10040423 | DOI Listing |
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