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GHR-mutant pig derived from domestic pig and microminipig hybrid zygotes using CRISPR/Cas9 system. | LitMetric

AI Article Synopsis

  • Pigs are valuable models for biomedical research due to their similarities to humans, but their size limits their use compared to smaller animals like rodents.
  • Researchers have created a mutant pig with a modified growth hormone receptor (GHR), leading to a smaller stature, specifically using a tiny breed called microminipig.
  • The study successfully produced biallelic GHR-mutant pigs and plans to backcross them with microminipigs to develop even smaller pigs for more effective research applications.

Article Abstract

Background: Pigs are excellent large animal models with several similarities to humans. They provide valuable insights into biomedical research that are otherwise difficult to obtain from rodent models. However, even if miniature pig strains are used, their large stature compared with other experimental animals requires a specific maintenance facility which greatly limits their usage as animal models. Deficiency of growth hormone receptor (GHR) function causes small stature phenotypes. The establishment of miniature pig strains via GHR modification will enhance their usage as animal models. Microminipig is an incredibly small miniature pig strain developed in Japan. In this study, we generated a GHR mutant pig using electroporation-mediated introduction of the CRISPR/Cas9 system into porcine zygotes derived from domestic porcine oocytes and microminipig spermatozoa.

Methods And Results: First, we optimized the efficiency of five guide RNAs (gRNAs) designed to target GHR in zygotes. Embryos that had been electroporated with the optimized gRNAs and Cas9 were then transferred into recipient gilts. After embryo transfer, 10 piglets were delivered, and one carried a biallelic mutation in the GHR target region. The GHR biallelic mutant showed a remarkable growth-retardation phenotype. Furthermore, we obtained F1 pigs derived from the mating of GHR biallelic mutant with wild-type microminipig, and GHR biallelic mutant F2 pigs through sib-mating of F1 pigs.

Conclusions: We have successfully demonstrated the generation of biallelic GHR-mutant small-stature pigs. Backcrossing of GHR-deficient pig with microminipig will establish the smallest pig strain which can contribute significantly to the field of biomedical research.

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Source
http://dx.doi.org/10.1007/s11033-023-08388-3DOI Listing

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