[The Distribution and Significance of Activated T Cells and Lymphocyte Subsets in Myelodysplastic Syndrome].

Objective: To investigate the distribution of bone marrow lymphocyte subsets in patients with myelodysplastic syndrome(MDS),the proportion of activated T cells with immunophenotype CD3HLA-DR in the lymphocytes and its clinical significance, and to understand the effects of different types of MDS, different immunophenotypes, and different expression levels of on the proportion of lymphocyte subsets and activated T cells.

Methods: The immunophenotypes of 96 MDS patients, the subsets of bone marrow lymphocytes and activated T cells were detected by flow cytometry. The relative expression of was detected by real-time fluorescent quantitative PCR, and the first induced remission rate (CR1) was calculated, the differences of lymphocyte subsets and activated T cells in MDS patients with different immunophenotype, different expression, and different course of disease were analyzed.

Results: The percentage of CD4T lymphocyte in MDS-EB-2, IPSS high-risk, CD34 cells >10%, and patients with CD34CD7 cell population and gene overexpression at intial diagnosis decreased significantly (<0.05), and the percentage of NK cells and activated T cells increased significantly (<0.05), but there was no significant difference in the ratio of B lymphocytes. Compared with the normal control group, the percentage of NK cells and activated T cells in IPSS-intermediate-2 group was significantly higher(<0.05), but there was no significant difference in the percentage of CD3T, CD4T lymphocytes. The percentage of CD4T cells in patients with complete remission after the first chemotherapy was significantly higher than in patients with incomplete remission(<0.05), and the percentage of NK cells and activated T cells was significantly lower than that in patients with incomplete remission (<0.05).

Conclusion: In MDS patients, the proportion of CD3T and CD4T lymphocytes decreased, and the proportion of activated T cells increased, indicating that the differentiation type of MDS is more primitive and the prognosis is worse.