Background: Retinal oxygen saturation (sO) provides essential information about the eye's response to pathological changes that can result in vision loss. Visible-light optical coherence tomography (vis-OCT) is a noninvasive tool that has the potential to measure retinal sO in a clinical setting. However, its reliability is currently limited by unwanted signals referred to as spectral contaminants (SCs), and a comprehensive strategy to isolate true oxygen-dependent signals from SCs in vis-OCT is lacking.
Methods: We develop an adaptive spectroscopic vis-OCT (ADS-vis-OCT) technique that can adaptively remove SCs and accurately measure sO under the unique conditions of each vessel. We also validate the accuracy of ADS-vis-OCT using ex vivo blood phantoms and assess its repeatability in the retina of healthy volunteers.
Results: In ex vivo blood phantoms, ADS-vis-OCT agrees with a blood gas machine with only a 1% bias in samples with sO ranging from 0% to 100%. In the human retina, the root mean squared error between sO values in major arteries measured by ADS-vis-OCT and a pulse oximeter is 2.1% across 18 research participants. Additionally, the standard deviations of repeated ADS-vis-OCT measurements of sO values in smaller arteries and veins are 2.5% and 2.3%, respectively. Non-adaptive methods do not achieve comparable repeatabilities from healthy volunteers.
Conclusions: ADS-vis-OCT effectively removes SCs from human images, yielding accurate and repeatable sO measurements in retinal arteries and veins with varying diameters. This work could have important implications for the clinical use of vis-OCT to manage eye diseases.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126115 | PMC |
| http://dx.doi.org/10.1038/s43856-023-00288-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Macrocyclic Diterpenoids from Possessing Activity Towards Autophagic Flux.
Int J Mol Sci
December 2024
State Key Laboratory of Functions and Applications of Medicinal Plants & College of Pharmacy, Guizhou Provincial Engineering Technology Research Center for Chemical Drug R&D, Guizhou Medical University, Guiyang 550014, China.
Euphjatrophanes H-L (-), four new jatrophane-type and one new lathyrane-type diterpenoid, were isolated from , along with eight known diterpenoids (-). Their structures were established on the basis of extensive spectroscopic analysis and X-ray crystallographic experiments. All compounds were subjected to bioactivity evaluation using flow cytometry in autophagic flux assays with HM mCherry-GFP-LC3 cells, the human microglia cells which stably expressed the tandem monomeric mCherry-GFP-tagged LC3.
View Article and Find Full Text PDFNear-infrared fluorescent probe for visualization of nitroxyl in the plant response to stress.
Anal Chim Acta
January 2025
College of Tobacco Science, Henan Agricultural University, Zhengzhou, 450046, China. Electronic address:
Background: Nitroxyl (HNO) is an emerging signaling molecule that plays a significant regulatory role in various aspects of plant biology, including stress responses and developmental processes. However, understanding the precise actions of HNO in plants has been challenging due to the absence of highly sensitive and real-time in situ monitoring tools. Consequently, it is crucial to develop effective and accurate detection methods for HNO.
View Article and Find Full Text PDFArticle Synopsis
- Nanocatalytic medicine aims to improve cancer treatment by developing nanotechnologies that selectively target tumor cells while sparing normal cells.
- The proposed solution involves using pH- and redox-responsive ferrocene-containing polymersomes (FcPsomes) that can control radical production and release therapeutic molecules based on their environment.
- These FcPsomes can facilitate a synergistic treatment approach by producing reactive oxygen species through the Fenton reaction, allowing for targeted and safe therapies that adapt to the tumor's conditions.
Tailoring Acid-Salt Hybrid Electrolyte Structure for Stable Proton Storage at Ultralow Temperature.
Adv Mater
December 2024
Hefei National Research Center for Physical Sciences at the Microscale, Department of Materials Science and Engineering, CAS Key Laboratory of Materials for Energy Conversion, University of Science and Technology of China, Hefei, Anhui, 230026, P. R. China.
The critical challenges in developing ultralow-temperature proton-based energy storage systems are enhancing the diffusion kinetics of charge carriers and inhibiting water-triggered interfacial side reactions between electrolytes and electrodes. Here an acid-salt hybrid electrolyte with a stable anion-cation-HO solvation structure that realizes unconventional proton transport at ultralow temperature is shown, which is crucial for electrodes and devices to achieve high rate-capacity and stable interface compatibility with electrodes. Through multiscale simulations and experimental investigations in the electrolyte employing ZnCl introduced into 0.
View Article and Find Full Text PDFFoam-based antibacterial hydrogel composed of carboxymethyl cellulose/polyvinyl alcohol/cerium oxide nanoparticles for potential wound dressing.
Int J Biol Macromol
December 2024
Department of Chemistry, Faculty of Science and Letters, Istanbul Technical University, Maslak, Istanbul, Turkey. Electronic address:
Foam-based wound dressing materials produced by dispersing gas phases in a polymeric material are soft, adapt to the body shape, and allow the absorption of wound exudate due to their porous structure. Most of these formulations are based on synthetic substances such as polyurethane. However, biopolymers have entered the field as a new player thanks to their biocompatible and sustainable nature.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!