AI Article Synopsis
- Innate immune responses help control SARS-CoV-2 replication, and a specific lactic acid bacteria strain, Lactococcus lactis strain Plasma (LC-Plasma), can activate immune cells called plasmacytoid dendritic cells (pDCs).
- Research showed that when pDCs are stimulated with LC-Plasma, their secretions can reduce SARS-CoV-2 replication in cell cultures.
- The study also found that the secretion of interferon-α (IFN-α) from pDCs is crucial to this suppression; blocking IFN-α decreased the effectiveness of LC-Plasma in reducing the virus's replication.
Article Abstract
Innate immune responses are important in the control of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication. We have previously found a lactic acid bacteria species, Lactococcus lactis strain Plasma (LC-Plasma), which possesses specific feature to activate plasmacytoid dendritic cells (pDCs) and thus may affect innate immune responses. Here, we investigated the impact of pDC activation by LC-Plasma on SARS-CoV-2 replication in vitro. Addition of the culture supernatant of pDCs stimulated with LC-Plasma resulted in suppression of SARS-CoV-2 replication in Vero and Calu-3 cells. We confirmed interferon-α (IFN-α) secretion in the supernatant of pDCs stimulated with LC-Plasma and induction of IFN-stimulated genes in cells treated with the pDC supernatant. Anti-IFN-α antibody impaired the suppression of SARS-CoV-2 replication by the supernatant of LC-Plasma-stimulated pDCs, suggesting that IFN-α plays an important role in the SARS-CoV-2 suppression. Our results indicate the potential of LC-Plasma to induce inhibitory responses against SARS-CoV-2 replication through pDC stimulation with IFN-α secretion.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10110276 | PMC |
| http://dx.doi.org/10.1016/j.bbrc.2023.04.046 | DOI Listing |
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