Novel Methyltransferase Regulated by CsMADSL1 Specifically Methylates EGCG in Cultivar "GZMe4".

Epigallocatechin-3--(4--methyl)gallate (EGCG4″Me) in possesses numerous beneficial biological activities. However, the germplasm rich in EGCG4″Me and the -methyltransferase responsible for EGCG4″Me biosynthesis are poorly understood. Herein, the content of EGCG3″Me and EGCG4″Me in the shoots of 13 cultivars was analyzed to demonstrate that EGCG4″Me is characteristically accumulated in the "GZMe4" cultivar but not in the other 12 cultivars. A novel -methyltransferase (CsOMTL1) was identified from "GZMe4" using RNA-Seq and correlation analysis. Using the recombinant enzyme, EGCG4″Me was synthesized in vitro. Overexpression of via Agrobacterium-mediated genetic transformation caused constitutive accumulation of EGCG4″Me in callus. Moreover, the transcription factor CsMADSL1 localized in the nucleus activated the transcription of and specifically interacted with its promoter. Hence, our study identified a novel -methyltransferase that characteristically catalyzes the synthesis of EGCG4″Me and a positive regulator of EGCG4″Me synthesis in "GZMe4", which might provide a strategy for the breeding of a tea cultivar rich in EGCG4″Me.