Studies of the high-grade serous ovarian cancer (HGSOC) tumor microenvironment, the most lethal gynecological cancer, aim to enhance the efficiency of established therapies. Cell motility is an important process of anti-tumor response. Using human and mouse HGSOC tumor slices combined with time-lapse imaging, we assessed the motility of CD8 T and myeloid cells. We developed a semi-supervised analysis of cell movements, identifying four cell behaviors: migrating, long migrating, static, and wobbling. Tumor slices were maintained 24h , retaining viability and cell movements. treatments with lipopolysaccharide altered CD8 T and myeloid cell behavior. chemotherapy reduced cell movements in human and mouse tumors and differentially affected CD8 T and myeloid cells in chemo-sensitive and chemo-resistant mouse models. tumor slices can extend mouse studies to human, providing a stepping stone to translate mouse cancer studies to clinical trials.
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| http://dx.doi.org/10.1016/j.isci.2023.106514 | DOI Listing |
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