Effect of Vicia sativa L. on Motility, Mortality and Expression Levels of hsp Genes in J2 Stage of Meloidogyne hapla.

Assuming that the seeds of L. have a stressful effect on J2 stage , we undertook research on the effect of these seeds on the motility and mortality of J2 and determined the expression levels of selected genes in J2. The assessment of the effect of seeds on the motility of specimens consisted of observing the movement of J2 immersed in a seed diffusate or in a tomato root filtrate at temperatures of 10, 17, and 21°C. In J2 treated with (cv. Ina) seed diffusates, the expression level of genes was determined by qPCR. J2 exposed to diffusates were found to lose their motility, while their mortality did not exceed 30%. J2 in the seed diffusate were characterized by an increase in the expression levels of the 90, 1, and 43 genes. It is suggested that the 90 gene may be a potential bioindicator of the environmental impact on nematodes. The impaired ability to move in J2 of is attributable to the occurrence of seeds in their habitat. These studies may contribute to developing methods of reducing crop damage caused by .