Extracellular vesicles (EVs) produced by adipocytes and other adipose tissue (AT) cells are present in the space between cells in the tissue and in circulation. These EVs have been shown to robustly signal between cells in the tissue and in distal organs. AT has unique biophysical properties that require an optimized EV isolation protocol that ensures an uncontaminated EV isolate. This protocol can be used to isolate and characterize the total, heterogeneous population of EVs from the AT.
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