Fluorescence quenching of 1-acyl-2-[6[(7 nitro-2,1,3-benzoxadiazol-4yl) amino]caproyl] phosphatidyl choline in small unilamellar vesicles consisting of phosphatidyl serine has been used to monitor the lipid phase separation induced by Zn2+ and Ca2+. Phase separation of vesicle membranes was observed with Zn2+ at concentrations as low as 125 microM. Low concentrations of Zn2+ required long incubation times to reach maximal quenching (120 minutes at 375 microM). When low concentrations of Ca2+ were added to the preparation during the developing phase of Zn2+-induced quenching, an explosive increase in fluorescence quenching was instantenously observed. Phase separation induced by sub-millimolar concentrations of Ca2+ could be increased at least 4 times when vesicles were pre-incubated with 250 microM of Zn2+.

Download full-text PDF

Source
http://dx.doi.org/10.1016/0006-291x(86)90465-1DOI Listing

Publication Analysis

Top Keywords

phase separation
16
concentrations zn2+
8
phosphatidyl serine
8
fluorescence quenching
8
separation induced
8
microm low
8
low concentrations
8
concentrations ca2+
8
zn2+
5
phase
5

Similar Publications

A novel pH-responsive full-bio-based surfactant (Ca-S) containing a dynamic covalent bond is synthesized using renewable cashew phenol, 5-chloro-2-furanaldehyde, and taurine. The structure of Ca-S is characterized by Fourier transform infrared spectroscopy (FTIR) and H nuclear magnetic resonance (NMR) analysis. Limonene containing oil-in-water (O/W) microemulsions are prepared on the basis of the Ca-S surfactant and are applied to the remediation of oil-contaminated soil under low-energy conditions at ambient temperature.

View Article and Find Full Text PDF

Many transcription factors (TFs) have been shown to bind to super-enhancers, forming transcriptional condensates to activate transcription in various cellular systems. However, the genomic and epigenomic determinants of phase-separated transcriptional condensate formation remain poorly understood. Questions regarding which TFs tend to associate with transcriptional condensates and what factors influence their association are largely unanswered.

View Article and Find Full Text PDF

Background: Incorporating β-carotene into food systems improves nutritional value by providing a natural source of vitamin A. However, maintaining its stability during processing and storage is a significant barrier for its bioavailability.

Results: This study investigated the utilization of banana rachis nanocellulose (BRNC) as a natural stabilizer in the formulation of Pickering nanoemulsion (PNE).

View Article and Find Full Text PDF

Asymmetric self-organization from a symmetric film by phase separation.

Nanoscale

January 2025

Department of Materials Science and Engineering, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata-ku, Kitakyushu 804-8550, Japan.

Self-organization realizes various nanostructures to control material properties such as superconducting vortex pinning and thermal conductivity. However, the self-organization of nucleation and growth is constrained by the growth geometric symmetry. To realize highly controlled three-dimensional nanostructures by self-organization, nanostructure formation that breaks the growth geometric symmetry thermodynamically and kinetically, such as tilted or in-plane aligned nanostructures, is a challenging issue.

View Article and Find Full Text PDF

The concentrations of individual proteins vary between cells, both developmentally and stochastically. The functional consequences of this variation remain largely unexplored due to limited experimental tools to manipulate the relationship of protein concentration to activity. Here, we introduce a genetically encoded tool based on a tunable amyloid that enables precise control of protein concentration thresholds in cells.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!