AI Article Synopsis
- The study focuses on yellow-green variegation in soybean leaves, a trait that increases value in ornamental plants but reduces crop yields.
- Researchers identified four mutants (Gmvar1 to Gmvar4) and found that a gene called GmCS1, which encodes a chorismate synthase, is responsible for this phenotype.
- Supplying specific aromatic amino acids like Phenylalanine to the mutants can reverse the variegation, highlighting changes in metabolic processes and gene regulation in these plants.
Article Abstract
Yellow-green variegation leaf phenotype adds more value to ornamental plants, but it is regarded as an undesirable trait in crop plants, affecting their yields. Until recently, the underlying mechanism regulating the yellow-green variegation phenotype has remained largely unexplored in soybean. In the present study, we indentified four Glycine max leaf yellow/green variegation mutants, Gmvar1, Gmvar2, Gmvar3, and Gmvar4, from artificial mutagenesis populations. Map-based cloning, together with the allelic identification test and CRISPR-based gene knockout, proved that mutated GmCS1 controls yellow-green variegation phenotype of the Gmvar mutants. GmCS1 encodes a chorismate synthase in soybean. The content of Phe, Tyr, and Trp were dramatically decreased in Gmcs1 mutants. Exogenous supply of three aromatic amino acid mixtures, or only Phe to Gmvar mutants, leads to recovery of the mutant phenotype. The various biological processes and signalling pathways related to metabolism and biosynthesis were altered in Gmvar mutants. Collectively, our findings provide new insights about the molecular regulatory network of yellow-green variegation leaf phenotype in soybean.
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| http://dx.doi.org/10.1093/jxb/erad139 | DOI Listing |
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