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In vitro prebiotic activity of rhLf and galactooligosaccharides on infant intestinal microbiota. | LitMetric

In vitro prebiotic activity of rhLf and galactooligosaccharides on infant intestinal microbiota.

Nutr Hosp

Departamento de Tecnología de los Alimentos, Nutrición y Bromatología. Facultad de Veterinaria. Campus de Excelencia Internacional "Campus Mare Nostrum". Universidad de Murcia.

Published: August 2023

AI Article Synopsis

  • The study investigates how human lactoferrin (Lf) and galactooligosaccharides (GOS) affect gut microbiota composition in a small-scale fermentation model using infant formula.
  • After 24 hours of fermentation, there were minimal changes in pH and short-chain fatty acids, but some bacterial groups, particularly Lactobacillus and Bifidobacterium, increased, indicating a potential prebiotic effect.
  • However, the results suggest that Lf's prebiotic activity may involve mechanisms beyond what can be measured in this fermentation model, as the protein nature of Lf complicates its detection as a prebiotic.

Article Abstract

Objective: human lactoferrin (Lf) and human milk oligosaccharides possess a wide range of functions. So, the present study focusses on the role of Lf and/or galactooligosaccharides (GOS) in the modulation of gut microbiota composition. Methods: recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model. Short-chain fatty acids (SCFAs), microbial population groups, and pH were monitored through fermentation for 24 hours. Results: insignificant changes were observed in pH values and acetic acid accumulated during fermentation. Propionic acid content has been insignificantly increased while butyric acid has been insignificantly decreased. Moreover, increments in all bacterial groups except for Bacteroides were observed through the fermentation process. Lactobacillus and Bifidobacterium showed an increase in relation to initial time over the fermentation process, demonstrating the prebiotic effect of lactoferrin and GOS. After 24 hours of fermentation, all tested ingredients showed significant similarities in Enterococcus for controls except for 0.20 % rhLf + 1 % GOS, which provoked a diminution of Enterococci growth. Conclusion: despite the importance of the batch culture fermentation technique in uncovering the prebiotic activity of food ingredients, it is not useful for detecting the prebiotic nature of Lf due to its nature as a protein. Thus, Lf maybe shows its prebiotic activity on the gut microbiota through other mechanisms.

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Source
http://dx.doi.org/10.20960/nh.04366DOI Listing

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