Automatic NMR-based protocol for assessment of honey authenticity.

Food Chem

Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, the Netherlands.

Published: September 2023

AI Article Synopsis

  • H NMR analysis helps confirm honey's plant origin, utilizing specific signals for various floral types and metabolites from bees.
  • A new method measures two parameters, entomological factor (EF) and aromatic factor (AF), to quickly detect honey adulteration without detailed NMR analysis.
  • This approach successfully identified all adulterated honey samples and revealed non-genuine honey that passed official methods, highlighting its effectiveness.

Article Abstract

H NMR analysis of organic extracts of honey is a powerful technique to confirm its botanical origin, thanks to the presence of signals that are specific to each floral typology. Similarly, signals from bee metabolites provide an important tool to verify honey entomological origin. Here, we present a method for honey screening that does not require any detailed analysis of the NMR spectrum for the detection and quantification of such markers. Our approach is based on the measurement of two spectral parameters, named entomological factor (EF) and aromatic factor (AF), calculated by integration of well-defined regions of the NMR spectrum. The values of EF and AF can reveal direct or indirect dilution of honey with sugar syrups. This method was tested on honeys of different floral origins and could identify all adulterated samples previously recognized by official techniques. Notably, several samples found compliant by official methods were proven non-genuine by the proposed approach.

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Source
http://dx.doi.org/10.1016/j.foodchem.2023.136094DOI Listing

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