gen. nov., sp. nov., a novel taxon within the family isolated from sludge.

A Gram-stain-negative bacterium, designated strain LB-8, was isolated from an activated sludge sample collected from a factory in Binzhou city, Shandong province, PR China. Cells of strain LB-8 were strictly aerobic, non-motile and rod-shaped. Growth occurred at 15-40 °C (optimum, 30 °C), at pH 6.0-9.0 (optimum, pH 7.0) and at 0-7.5 % (w/v) NaCl (optimum, 0.5 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LB-8 formed a distinct phyletic branch within the family and was most closely related to members of the genera , and with 92.7-93.3 % 16S rRNA gene sequence similarities. The average amino acid identity values between strain LB-8 and its closed phylogenetic neighbours , and were below 70 % supporting that strain LB-8 was a member of a novel genus. The predominant cellular fatty acids of LB-8 were iso-C, anteiso-C, summed feature 3 (C 7 and/or C 6) and iso-C 3-OH, and the only isoprenoid quinone was menaquinone-7 (MK-7). The major polar lipids of strain LB-8 were phosphatidylethanolamine, four unidentified aminolipids and two unidentified lipids. The genome size of strain LB-8 was 7.01 Mbp with 41.2 mol% G+C content. On the basis of the evidence presented in this study, strain LB-8 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. (type strain LB-8=GDMCC 1.3631=KCTC 92688) is proposed.