Toll-Like Receptor 7 a Novel Non-Invasive Inflammatory Genetic Sensor for Ulcerative Colitis Remission Monitoring.

Background: Ulcerative colitis (UC) and Crohn's disease (CD) are two major types of inflammatory bowel diseases (IBDs). Toll-like receptors (TLRs) are expressed in the innate immune system compartments, in charge of identifying a wide range of microorganisms. The aim of the present study was to evaluate the expression of , -, and - in peripheral blood mononuclear cells (PBMC) of UC patients as a novel non-invasive primary inflammation sensor for monitoring the clinical course of UC candidates.

Materials And Methods: In this cross-sectional study, total RNA was extracted from the PBMC of 42 UC patients along with 20 healthy donors. The mRNA levels of , -, and - were assessed using the quantitative real-time polymerase chain (qRT-PCR) reaction.

Results: The present research study demonstrated no significant changes in mRNA expression in UC patients in comparison with the control group ( = 0.1264), whereas significant elevation ( = 0.0008) was distinguished in the expression of UC participants specifically during the remission course compared with healthy donors and flareup patients ( = 0.0004 and = 0.0063, respectively). The last selected TLR, was not shown remarkable changes either between UC patients and the control group or between clinical courses of the disease.

Conclusion: Here, among three nominated TLRs for predicting UC patients, -7 was potentially selected according to the significant difference in mRNA expression in flareup UC patients and control donors. -7 could be used as a novel non-invasive biomarker for monitoring UC patients in the active course of the disease.