AI Article Synopsis
- Plant surface properties are vital for coping with environmental stresses, and the cuticle's outer layer is made up of diverse waxes influenced by specific gene regulators.
- In this study, researchers used a technique called RNA-guided Cas9 endonuclease to create mutations in four barley genes linked to cuticle properties, identifying one gene responsible for a glossy sheath phenotype due to a deficiency in β-diketones.
- The results indicated that the WIN1 transcription factor plays a crucial role in regulating the biosynthesis of β-diketones in barley by activating certain genes during specific developmental stages and in particular plant organs.
Article Abstract
Plant surface properties are crucial determinants of resilience to abiotic and biotic stresses. The outer layer of the plant cuticle consists of chemically diverse epicuticular waxes. The WAX INDUCER1/SHINE subfamily of APETALA2/ETHYLENE RESPONSIVE FACTORS regulates cuticle properties in plants. In this study, four barley genes homologous to the gene were mutated using RNA-guided Cas9 endonuclease. Mutations in one of them, the gene, caused a recessive glossy sheath phenotype associated with β-diketone deficiency. A complementation test for knockout (KO) and mutants showed that and are allelic variants of the same genomic locus. A comparison of the transcriptome from leaf sheaths of KO and wild-type plants revealed a specific and strong downregulation of a large gene cluster residing at the previously known locus. Our findings allowed us to postulate that the WIN1 transcription factor in barley is a master mediator of the β-diketone biosynthesis pathway acting through developmental stage- and organ-specific transactivation of the gene cluster.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095013 | PMC |
| http://dx.doi.org/10.3390/ijms24076762 | DOI Listing |
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