AI Article Synopsis
- - The analysis of tissue alterations is essential in pathology for accurate diagnoses, and new techniques in tissue clearing and fluorescence microscopy have enabled 3D studies of biological tissues.
- - Combining advanced fluorescence microscopy with classical staining methods like H&E has led to the development of 3D histology, allowing for more detailed observations of tissue structures.
- - The use of fluorescence staining techniques allows for comparable results to traditional methods, making 3D imaging accessible for pathologists and opening up new possibilities in clinical pathology.
Article Abstract
The analysis of histological alterations in all types of tissue is of primary importance in pathology for highly accurate and robust diagnosis. Recent advances in tissue clearing and fluorescence microscopy made the study of the anatomy of biological tissue possible in three dimensions. The combination of these techniques with classical hematoxylin and eosin (H&E) staining has led to the birth of three-dimensional (3D) histology. Here, we present an overview of the state-of-the-art methods, highlighting the optimal combinations of different clearing methods and advanced fluorescence microscopy techniques for the investigation of all types of biological tissues. We employed fluorescence nuclear and eosin Y staining that enabled us to obtain hematoxylin and eosin pseudo-coloring comparable with the gold standard H&E analysis. The computational reconstructions obtained with 3D optical imaging can be analyzed by a pathologist without any specific training in volumetric microscopy, paving the way for new biomedical applications in clinical pathology.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094801 | PMC |
| http://dx.doi.org/10.3390/ijms24076747 | DOI Listing |
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