Although several regulators associated with purple traits in rice have been identified, the genetic basis of the purple sheath remains unclear. In the present study, F2-1 and F2-2 populations were constructed using purple sheath (H93S) and green sheath (R1173 and YHSM), respectively. In order to identify QTL loci in purple sheaths, BSA analyses were performed on the two F2 populations. A crucial QTL for purple sheath was identified, tentatively named qPLSr6, and was located in the 4.61 Mb to 6.03 Mb region of chromosome 6. Combined with expression pattern analysis of candidate genes, () was suggested as a candidate gene. The homozygous mutant KO-1 and KO-2 created through CRISPR/Cas9 editing, lost their purple leaf sheath. The RT-PCR revealed that , anthocyanin synthase (ANS), diflavonol-4-reductase (DFR), flavanone-3-hydroxylase (F3H), and flavanone-3'-hydroxylase (F3'H) expression levels were dramatically down-regulated in the mutants. The yeast report system indicated that the 145-272 aa region at the C-terminal of OsC1 is a positive transcriptional activation domain. The results indicated that OsC1 synthesized anthocyanins by regulating the expression of ANS, DFR, F3H, and F3'H. This study provides new insights into the genetic basis of the purple sheath.
Plant Biology Division, Institute of Biological Sciences, College of Arts and Sciences, Central Mindanao University, University Town, Musuan, Maramag, Bukidnon 8714, Philippines.
A new species of is described and illustrated. This species is very similar to (sheathing and leaf morphology) but differs by the inflorescence and flowers. It is closely related also to by the deep purple inflorescence, but differs in having longer peduncle, broadly ovate-concave to cucullate spathe (which is deep wine-red when fresh to purplish-black when senescing), and the spadix that is 7/10 the entire length of the spathe.
Bacterial panicle blight (BPB) is one of the emerging diseases occurring in different Agro-Ecological Zones (AEZ) of Bangladesh and can cause up to 75% yield loss. In Bangladesh, the typical symptoms of BPB include sheath rot, panicle blight, grain spotting, and grain rot in both inbred and hybrid rice varieties, which resemble those reported by Zhou (2019). To confirm, 300 field samples of 20 panicles each with typical BPB symptoms from 20 districts (3 locations each district and 5 fields per location) were collected during mid-November 2022 for the causal pathogen(s) isolation.
- A significant outbreak of bacterial pith necrosis in tomatoes occurred in August 2023 in Yunnan Province, China, affecting over 40% of the plants in a single greenhouse, causing symptoms like waterlogged rot and wilting of leaves.
- The pathogen was isolated using various microbiological techniques, including disinfection, tissue immersion, and culturing on specific agar media, leading to the identification of a strain called Kv4.
- Biochemical tests and DNA sequencing revealed strain Kv4’s characteristics, aligning it closely with a known species, confirming its identity through high sequence similarity in the 16S rDNA and other genes.
Fluorescent whitening agents (FWAs) are dyes that emit visible blue or blue-purple fluorescence upon ultraviolet-light absorption. Taking advantage of light complementarity, FWAs can compensate for the yellow color of many substances to achieve a whitening effect; thus, they are used extensively in various applications. FWAs are generally stable, but their presence in the environment can lead to pollution and accumulation in the body through the food chain.
We identified a SbPLSH1gene conferring purple leaf sheath in sorghum (sorghumbicolor(L.) Moench)and developed a functional markerfor it. The purple leaf sheath of sorghum, a trait mostly related to anthocyanin deposition, is a visually distinguishable morphological marker widely used to evaluate the purity of crop hybrids.
