AI Article Synopsis
- The plant studied is part of the Campanulaceae family and is significant for its medicinal and dietary uses in East Asia, yet its genome evolution and biochemical pathways remain largely unclear.
- A comprehensive chromosome-scale genome assembly was achieved using advanced sequencing and capture techniques, resulting in a genome size of 574 Mb with a high completeness level of 97.6%.
- The research identified 41,355 protein-coding genes and revealed an increase in 14 genes linked to triterpenoid saponin production, paving the way for improved breeding methods to enhance these valuable compounds.
Article Abstract
belongs to the Campanulaceae family and is an important medicinal and food plant in East Asia. However, on the whole, the genome evolution of and the molecular basis of its major biochemical pathways are poorly understood. We reported a chromosome-scale genome assembly of based on a hybrid method using Oxford Nanopore Technologies, Illumina sequences, and high-throughput chromosome conformation capture (Hi-C) analysis. The assembled genome was finalized as 574 Mb, containing 41,355 protein-coding genes, and the genome completeness was assessed as 97.6% using a Benchmarking Universal Single-Copy Orthologs analysis. The genome comprises nine pseudo-chromosomes with 56.9% repeat sequences, and the transcriptome analysis revealed an expansion of the 14 genes related to triterpenoid saponin biosynthesis. Our findings provide an understanding of genome evolution and enable genomic-assisted breeding for the mass production of important components such as triterpenoid saponins.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095269 | PMC |
| http://dx.doi.org/10.3390/ijms24076534 | DOI Listing |
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