AI Article Synopsis
- * The study introduces a novel nanocomplex, Pdot-siRNA, made from semiconductor polymer dots (Pdots) that can effectively target and down-regulate specific genes in female germline stem cells (FGSCs) with minimal impact on their differentiation.
- * The Pdots exhibit high fluorescence for better tracking, escape lysosomes, and are able to penetrate into 3D ovarian organoids, highlighting their potential as efficient nanocarriers for therapeutic applications in managing FGSCs.
Article Abstract
Germline stem cells (GSCs) are the only cell population capable of passing genetic information to offspring, making them attractive targets in reproductive biology and fertility research. However, it is generally more difficult to introduce exogenous biomolecules into GSCs than other cell types, impeding the exploration and manipulation of these cells for biomedical purposes. Herein, semiconductor polymer dots (Pdots)-based nanocomplex Pdot-siRNA is developed and achieves effective knockdown of target genes in female germline stem cells (FGSCs). Advantage of high fluorescence brightness of Pdots is taken for comprehensive investigation of their cellular uptake, intracellular trafficking, and exocytosis in FGSCs. Importantly, Pdots show excellent biocompatibility and minimally disturb the differentiation of FGSCs. Intracellular Pdots escape from the lysosomes and undergo active exocytosis, which makes them ideal nanocarriers for bioactive cargos. Moreover, Pdot-siRNA can penetrate into 3D ovarian organoids derived from FGSCs and down-regulate the expression levels of target genes. This study investigates the interface between a type of theranostic nanoparticles and FGSCs for the first time and sheds light on the manipulation and medical application of FGSCs.
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| http://dx.doi.org/10.1002/adma.202210458 | DOI Listing |
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