Morphofunctional Features of Microglial Cells during the Administration of Orexin A.

The neurotransmitter orexin A (1 μl of a solution with a concentration of 0.3 mM) was injected into the second brain ventricle of wild-type C57BL/6 mice; 1 h later, LPS was injected intraperitoneally at a dose of 2 mg/kg to activate microglial cells. Administration of orexin A attenuated activation of microglial cells assessed by changes in the length of filopodia. Injection of orexin was followed by an increase in the length of the processes, which indicates a decrease in activity of microglial cells. Using double immunohistochemical staining, we found that the number of microglial cells and orexin A receptors on microglial cells increased after LPS injection. Our findings confirm the participation of orexin A in the modulation of the functional activity of microglial cells.