Verticillium wilt is one of the most critical cotton diseases, which is widely distributed in cotton-producing countries. However, the conventional method of verticillium wilt investigation is still manual, which has the disadvantages of subjectivity and low efficiency. In this research, an intelligent vision-based system was proposed to dynamically observe cotton verticillium wilt with high accuracy and high throughput. Firstly, a 3-coordinate motion platform was designed with the movement range 6,100 mm × 950 mm × 500 mm, and a specific control unit was adopted to achieve accurate movement and automatic imaging. Secondly, the verticillium wilt recognition was established based on 6 deep learning models, in which the VarifocalNet (VFNet) model had the best performance with a mean average precision () of 0.932. Meanwhile, deformable convolution, deformable region of interest pooling, and soft non-maximum suppression optimization methods were adopted to improve VFNet, and the of the VFNet-Improved model improved by 1.8%. The precision-recall curves showed that VFNet-Improved was superior to VFNet for each category and had a better improvement effect on the ill leaf category than fine leaf. The regression results showed that the system measurement based on VFNet-Improved achieved high consistency with manual measurements. Finally, the user software was designed based on VFNet-Improved, and the dynamic observation results proved that this system was able to accurately investigate cotton verticillium wilt and quantify the prevalence rate of different resistant varieties. In conclusion, this study has demonstrated a novel intelligent system for the dynamic observation of cotton verticillium wilt on the seedbed, which provides a feasible and effective tool for cotton breeding and disease resistance research.
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http://dx.doi.org/10.34133/plantphenomics.0013 | DOI Listing |
Int J Mol Sci
December 2024
College of Agricultural, Tarim University, Alar 843300, China.
wilt (VW) caused by (Vd) is a devastating fungal cotton disease characterized by high pathogenicity, widespread distribution, and frequent variation. It leads to significant losses in both the yield and quality of cotton. Identifying key non-synonymous single nucleotide polymorphism (SNP) markers and crucial genes associated with VW resistance in and , and subsequently breeding new disease-resistant varieties, are essential for VW management.
View Article and Find Full Text PDFFungal Genet Biol
January 2025
Team of Crop Verticillium wilt, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji 831100, China. Electronic address:
The vascular wilt fungus Verticillium dahliae is a destructive soil-borne pathogen that causes yield loss on various economically important crops. Membrane-spanning sensor protein SLN1 have been demonstrated to contribute to virulence in varying degrees among numerous devastating fungal pathogens. However, the biological function of SLN1 in V.
View Article and Find Full Text PDFMicroorganisms
November 2024
College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010019, China.
Sunflower Wilt (SVW) caused by is a significant threat to sunflower production in China. This soilborne disease is difficult to control. It has been observed that delayed sowing reduces the severity of SVW on different varieties and across various locations.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
The Key Laboratory of Oasis Eco-Agriculture, Agriculture College, Shihezi University, Shihezi 832000, China.
is a soil-borne phytopathogenic fungus causing destructive Verticillium wilt disease that greatly threats cotton production worldwide. The mechanism of cotton resistance to Verticillium wilt is very complex and requires further research. In this study, RNA-sequencing was used to investigate the defense responses of cotton leaves using varieties resistant (Zhongzhimian 2, or Z2) or susceptible (Xinluzao 7, or X7) to .
View Article and Find Full Text PDFJ Fungi (Basel)
December 2024
College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010010, China.
, previously classified in the genus until 2007, is an attenuated pathogen known to provide cross-protection against wilt in various crops. To investigate the potential mechanisms underlying its reduced virulence, we conducted genome sequencing, annotation, and a comparative genome analysis of GnVn.1 (GnVn.
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