Pakistan Council of Scientific and Industrial Research, 01-Constitution Avenue, G-5/2, Islamabad 44050, Pakistan.
Published: April 2023
AI Article Synopsis
Article Abstract
Urease enzyme is an infectious factor that provokes the growth and colonization of virulence pathogenic bacteria in humans. To overcome the deleterious effects of bacterial infections, inhibition of urease enzyme is one of the promising approaches. The current study is designed to synthesize new 1,2-benzothiazine--arylacetamide derivatives () that can effectively provide a new drug candidate to avoid bacterial infections by urease inhibition. After structural elucidation by FT-IR, proton and carbon-13 NMR and mass spectroscopy, the synthesized compounds () were investigated to evaluate their inhibitory potential against urease enzyme. analysis against positive control of thiourea indicated that all the synthesized compounds have strong inhibitory strengths as compared to the reference drug. Compound , being the most potent inhibitor, strongly inhibited the urease enzymes and revealed an IC value of 9.8 ± 0.023 µM when compared with the IC of thiourea (22.3 ± 0.031 µM)-a far more robust inhibitory potential. Docking studies of within the urease active site revealed various significant interactions such as H-bond, π-alkyl with amino acid residues like Val744, Lys716, Ala16, Glu7452, Ala37 and Asp730.
Objectives: To delineate and understand the genetic variations among strains from Trinidad and Tobago associated with gastric diseases.
Methods: One hundred (n = 100) patients who routinely presented with clinical features suggestive of peptic disease were enrolled in the study and underwent gastroscopy procedures. Biopsy specimens were analyzed using serological and molecular methods.
Alfalfa ( L.) establishment is an effective strategy for grassland reconstruction in degraded ecosystems. However, the mechanisms underlying vegetation succession in reconstructed grasslands following alfalfa establishment remain elusive.
College of Chemistry and Chemical Engineering, Key Laboratory of Chemical Engineering and Technology of State Ethnic Affairs Commission, North Minzu University, Yinchuan, 750021, China.
Soil salinization poses a substantial threat to global food security, particularly under the influence of climate change, and is recognized as one of the most urgent challenges in land degradation. This study aims to elucidate the challenges associated with managing arid and semi-arid saline-alkali lands in China's Ningxia province and propose feasible solutions. To assess moss crust colonization, we measured changes in organic matter and chlorophyll levels.
Urea is a major issue in human wastewater because it may be easily broken down by the urease enzyme produced by bacteria, leading to ammonia production. Due to its ability to increase soil pH and eutrophicate streams, ammonia-containing effluent emissions pose environmental and health risks. This study aimed to evaluate the effectiveness of various treatment approaches in reducing urea concentrations by comparing the removal rates of conducting electrocoagulation (EC), EC followed by chemical coagulation (EC-CC), and CC followed by electrocoagulation (EC-CC).
Background: Casing soil is critical for the cultivation process of and promotes the formation of mushroom fruiting bodies. Therefore, reliable casing soil indicators are crucial for obtaining high yields of high-quality mushrooms.
Methods: In this study, soil enzyme activity, physicochemical properties, and microorganisms at five cultivation stages [namely casing (A1), mycelial (A2), primordial (A3), fruiting (A4), and harvesting (A5)] of cultivation were evaluated in casing soils.
