We present the complete genome sequences of 9 species of Papilionidae from 3 genera (Graphium, Ornithoptera, Papilio). Illumina sequencing was performed on genetic material from individual wild-caught specimens. The reads were assembled using a de novo method followed by a finishing step. The raw and assembled data are publicly available via Genbank.
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http://dx.doi.org/10.56179/001c.73927 | DOI Listing |
Bioengineered
December 2025
Department of BioMedical Bigdata (BK21) and Research Institute of Life Sciences, Gyeongsang National University, Jinju, Republic of Korea.
Gene editing is emerging as a powerful tool for introducing novel functionalities in mushrooms. While CRISPR/Cas9-induced double-strand breaks (DSBs) typically rely on non-homologous end joining (NHEJ) for gene disruption, precise insertion of heterologous DNA in mushrooms is less explored. Here, we evaluated the efficacy of inserting donor DNAs (8-1008 bp) with or without homologous arms at Cas9-gRNA RNP-induced DSBs.
View Article and Find Full Text PDFMicrobiol Resour Announc
January 2025
Centre de Biotechnologies Végétales et Microbiennes, Biodiversité et Environnement, Faculty of Sciences, Mohammed V University in Rabat, Rabat, Morocco.
In this study, we present the complete genome of LLZ14, a nodule-forming bacterium isolated from root nodules with high plant growth-promoting abilities. This genome contains genes predicted to be involved in plant stress tolerance and growth promotion, including auxin production, phosphatase, and 1-aminocyclopropane-1-carboxylate deaminase.
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January 2025
Malopolska Centre of Biotechnology, Jagiellonian University, Kraków, Poland.
Average nucleotide identity (ANI) is a widely used metric to estimate genetic relatedness, especially in microbial species delineation. While ANI calculation has been well optimized for bacteria and closely related viral genomes, accurate estimation of ANI below 80%, particularly in large reference data sets, has been challenging due to a lack of accurate and scalable methods. To bridge this gap, we introduce MANIAC, an efficient computational pipeline optimized for estimating ANI and alignment fraction (AF) in viral genomes with divergence around ANI of 70%.
View Article and Find Full Text PDFMicrobiol Resour Announc
January 2025
Laboratory of Molecular Virology, Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.
Human pegivirus (HPgV) identified from an HCV-infected plasma sample through nanopore metagenomics. The analysis revealed a nearly complete HPgV-2 genome. Phylogenetic analysis confirmed its classification within the HPgV-2 genotype, providing insights into viral co-infection dynamics.
View Article and Find Full Text PDFMicrobiol Resour Announc
January 2025
Graduate School of Environmental Studies, Tohoku University, Sendai, Japan.
This article reports on the complete genome of sp. strain Stari2, which was shown to have the ability to degrade carbon tetrachloride (CCl) in aerobic conditions. A single circular sequence of 6,310,573 bp, a GC content of 60.
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