AI Article Synopsis
- Reverse genetics is a crucial method for understanding viruses and creating vaccines, and this study utilizes a yeast-based platform to reconstruct viruses.
- A transformation-associated recombination (TAR) system was employed to quickly recover different strains of the feline infectious peritonitis virus, which is particularly dangerous for cats.
- The newly developed system allows for rapid and stable virus assembly, aiding in the study of feline coronaviruses and advancing vaccine research.
Article Abstract
Introduction: Reverse genetics has become an indispensable tool to gain insight into the pathogenesis of viruses and the development of vaccines. The yeast-based synthetic genomics platform has demonstrated the novel capabilities to genetically reconstruct different viruses.
Methods: In this study, a transformation-associated recombination (TAR) system in yeast was used to rapidly rescue different strains of feline infectious peritonitis virus, which causes a deadly disease of cats for which there is no effective vaccine.
Results And Discussion: Using this system, the viruses could be rescued rapidly and stably without multiple cloning steps. Considering its speed and ease of manipulation in virus genome assembly, the reverse genetics system developed in this study will facilitate the research of the feline coronaviruses pathogenetic mechanism and the vaccine development.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10076789 | PMC |
| http://dx.doi.org/10.3389/fmicb.2023.1141101 | DOI Listing |
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