The production of xylo-oligosaccharides (XOS) from lignocelluloses with conjugated acid-base system is highly efficient. However, XOS production from wheat straw with conjugated system of acetic acid/sodium acetate (HAc/NaAc) has not been reported. In addition, the impact of delignification of wheat straw for XOS production was not clear. In this study, the optimal conditions for HAc/NaAc hydrolysis were: 0.4 M, molar ratio 10:1, 170 °C, and 60 min. The yield of XOS could be rose to 50.2% after the HAc/NaAc hydrolysate was hydrolyzed using xylanase. After 70.3% of lignin in wheat straw was removed by hydrogen peroxide-acetic acid treatment, the XOS yield of delignified wheat straw using HAc/NaAc reached 54.7%. Finally, a glucose yield of 96.6% was gained by cellulase from wheat straw solid. This work suggested that HAc/NaAc hydrolysis could efficiently produce XOS from wheat straw and the delignification of wheat straw was conducive to XOS and monosaccharides production.
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