Direct Xylella fastidiosa whole genome sequencing from various plant species using targeted enrichment.

J Microbiol Methods

Anses, Plant Health Laboratory, Bacteriology Virology GMO Unit, 7 rue Jean Dixméras, 49044 Angers cedex 01, France.

Published: May 2023

AI Article Synopsis

  • A new method was created to directly sequence the DNA of Xylella fastidiosa from plant samples.
  • This method was tested on different plant species infected with various strains of the bacteria and at various contamination levels.
  • The results showed that the genome coverage of X. fastidiosa exceeded 99.9% in all samples tested.

Article Abstract

A targeted enrichment method was developed to sequence Xylella fastidiosa genomic DNA directly from plant samples. The method was evaluated on various plant species infected with different strains at different levels of contamination. After enrichment, X. fastidiosa genome coverage was above 99.9% for all tested samples.

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Source
http://dx.doi.org/10.1016/j.mimet.2023.106719DOI Listing

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