AI Article Synopsis
- Researchers are seeking efficient methods for detecting low levels of E. coli O157:H7 in various samples, but current methods can be complex or time-consuming.
- A new visual assay was developed using a stable enzyme-catalyzed system that can detect as few as 1 CFU/mL of E. coli.
- This method showed high selectivity and stability, successfully testing samples like milk and seawater, with detection limits of 10 CFU/mL visible to the naked eye.
Article Abstract
Various methods have been made to achieve sensitive detection (10 CFU/mL) of Escherichia coli O157:H7 (E. coli) in real samples, however, they are complex, time-consuming, or instrument-dependent. Enzyme-catalyzed reactions are one of the most efficient methods to amplify signals for sensitive detection. ZIF-8 owning stability, porosity, and high specific area are suitable for embedding enzymes which can effectively protect enzyme activity and thus improve detection sensitivity. Herein, a simple visual assay of E. coli with the limits of detection of 1 CFU/mL was developed based on this stable enzyme-catalyzed amplified system. A microbial safety test of milk, orange juice, seawater, cosmetic, and hydrolyzed yeast protein, was successfully performed with the limits of detection of 10 CFU/mL by the naked eye. And this bioassay possessed high selectivity and stability making the developed detection method practically promising.
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| http://dx.doi.org/10.1016/j.talanta.2023.124503 | DOI Listing |
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