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http://dx.doi.org/10.1016/j.pdpdt.2023.103553 | DOI Listing |
Biomacromolecules
January 2025
Institute of Surface Engineering (IOM), Permoserstraße 15, 04318 Leipzig, Germany.
Hybrid hydrogels are promising for wound dressing, tissue engineering, and drug delivery due to their exceptional biocompatibility and mechanical stability. This study synthesized hybrid hydrogels for photodynamic therapy using electron beam-initiated polymerization with varying PEGDA/gelatin ratios and irradiation doses to evaluate their effectiveness as uptake and release systems for five photosensitizers. Toluidine blue, O (TBO); methylene blue (MB); eosin, Y; indocyanine, green; and sodium meso-tetraphenylporphine-4,4',4″,4‴-tetrasulfonate were studied for their uptake and release dynamics in relation to their structural properties and the hydrogels' composition.
View Article and Find Full Text PDFInt J Mol Sci
November 2024
Chair of Chemistry, The Institute of Pharmacy, Sechenov First Moscow State Medical University (Sechenov University), 119571 Moscow, Russia.
Combined viral and photodynamic therapy for oncological diseases has great potential to treat aggressive tumors such as glioblastomas. A conjugate of vesicular stomatitis virus (VSV) with protoporphyrin IX was prepared, and its oncolytic effects were studied and compared to the effects of the individual components. The VSV showed an oncolytic effect on glioblastoma cell lines T98G and LN229 at a virus titer of 10 TCID/mL.
View Article and Find Full Text PDFPhotodiagnosis Photodyn Ther
December 2024
Institute for Physics and Engineering in Biomedicine, National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Moscow 115409, Russia.
The problem of treating cancer patients with lung cancer has become more difficult due to the SARS-CoV-2 viral infection and concomitant bacterial lesions. The analysis shows that the photodynamic effect of long-wavelength polycationic photosensitizers suppresses the tumor process (including the destruction of cancer stem cells), SARS-CoV-2 coronavirus infection, Gram-positive and Gram-negative bacteria, including those that can cause pneumonia. Therefore, the photodynamic approach using such photosensitizers is promising for the development of an effective treatment method for patients with lung cancer, including those with SARS-CoV-2 infection and bacterial complications.
View Article and Find Full Text PDFBiol Methods Protoc
November 2024
Faculty of Natural Sciences, Department of Microbiology and Virology, Comenius University in Bratislava, Bratislava, 84215, Slovakia.
Determining the number of viable cells by calculating colony-forming units is time-consuming. The evaluation of mixed biofilms consisting of different species is particularly problematic. Therefore, the aim of this study was to optimize a molecular method-propidium monoazide quantitative polymerase chain reaction (PMA-qPCR)-for accurate and consistent differentiation between living and dead cells.
View Article and Find Full Text PDFClin Res Hepatol Gastroenterol
November 2024
Department of Pathology, College of Medicine, King Khalid University, Abha, 62529, Saudi Arabia.
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