AI Article Synopsis

  • Genome editing, especially techniques like CRISPR/Cas, TALENs, and ZFNs, is increasingly important for functional genomics and improving crop traits.
  • These methods allow for rapid and precise modification of genetic material, aiding both plant research and breeding practices.
  • The review emphasizes CRISPR/Cas9's role in enhancing crop characteristics and discusses strategies for efficiently editing related gene families.

Article Abstract

Genome editing is a useful, adaptable, and favored technique for both functional genomics and crop enhancement. Over the years, rapidly evolving genome editing technologies, including clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas), transcription activator-like effector nucleases (TALENs), and zinc finger nucleases (ZFNs), have shown broad application prospects in gene function research and improvement of critical agronomic traits in many crops. These technologies have also opened up opportunities for plant breeding. These techniques provide excellent chances for the quick modification of crops and the advancement of plant science in the future. The current review describes various genome editing techniques and how they function, particularly CRISPR/Cas9 systems, which can contribute significantly to the most accurate characterization of genomic rearrangement and plant gene functions as well as the enhancement of critical traits in field crops. To accelerate the use of gene-editing technologies for crop enhancement, the speed editing strategy of gene-family members was designed. As it permits genome editing in numerous biological systems, the CRISPR technology provides a valuable edge in this regard that particularly captures the attention of scientists.

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Source
http://dx.doi.org/10.1007/s10142-023-01036-wDOI Listing

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