A self-designed device integrated with a Fermat spiral microfluidic chip for ratiometric and automated point-of-care testing of anthrax biomarker in real samples. | LitMetric
School of Mechanical Engineering, Yangzhou University, Yangzhou, 225127, China. Electronic address:
Published: June 2023
AI Article Synopsis
Article Abstract
A desirable lanthanide-based ratiometric fluorescent probe was designed and integrated into a self-designed Fermat spiral microfluidic chip (FS-MC) for the automated determination of a unique bacterial endospore biomarker, dipicolinic acid (DPA), with high selectivity and sensitivity. Here, a blue emission wavelength at 425 nm was generated in the Fermat spiral structure by mixing the europium (Eu) and luminol to form the Eu/Luminol sensing probe. DPA in the reservoir can be used to specifically bind to Eu under the negative pressure and transfer energy from DPA to Eu sequentially via an antenna effect, thus resulting in a significant increase in the red fluorescence emission peak at 615 nm. According to the fluorescence intensity ratio (F615/F425), a good linearity can be obtained with increasing the concentration of DPA from 0 to 200 μM with a limit of detection as low as 10.11 nM. Interestingly, the designed FS-MC can achieve rapid detection of DPA in only 1 min, reducing detection time and improving sensitivity. Furthermore, a self-designed device integrated with the FS-MC and a smartphone color picker APP was employed for the rapid automatic point-of-care testing (POCT) of DPA in the field, simplifying complex processes and reducing testing times, thus confirming the great promise of this ready-to-use measurement platform for in situ inspection.
Structured beams carrying orbital angular momentum (OAM) provide powerful capabilities for applications in optical tweezers, super-resolution imaging, quantum optics, and ad-vanced microparticle manipulation. However, it is challenging for generate and control the OAM beams at the extreme ultraviolet (EUV) region due to the lack of suitable wave front shaping optics arise from being limited to the strong absorption of most materials. Here, we use a modified Fermat-spiral photon-sieve splitter to simultaneously generate two focused doughnut beams with opposite helical phase.
Purpose: To develop a robust 3D ultrashort-TE (UTE) protocol that can reproducibly provide high-quality images, assessed by the ability to yield clinically diagnostic images, and is suitable for clinical translation.
Theory And Methods: Building on previous work, a UTE sampled with Fermat looped orthogonally encoded trajectories (FLORET) was chosen as a starting point due to its shorter, clinically reasonable scan times. Modifications to previous FLORET implementations included gradient waveform frequency limitations, a new trajectory ordering scheme, a balanced SSFP implementation, fast gradient spoiling, and full inline reconstruction.
* Current non-destructive testing methods struggle to identify small cracks due to their hidden nature and interference from dense thread signals.
* The proposed ultrasonic phased array method using a Fermat spiral arrangement improves detection by enhancing imaging accuracy and energy, benefiting the safety of wind turbine components and the wind power industry.
This paper introduces a structured beam with Archimedes spiral intensity distribution. The Archimedes spiral (AS) beam is the composite of a helical-axicon generated (HAG) Bessel beam and a Gaussian (GS) beam. We observed the spiral intensity patterns using computational holography, achieving the tuning over spiral arms number and spiral spacing.
The heliostat field layout in a central receiver solar thermal power plant has significant optical losses that can ultimately affect the overall output power of the plant. In this paper, an optimized heliostat field layout based on annual efficiency and power of 50 MW for the local coordinates of Quetta, Pakistan, is proposed. The performance of two different heliostat field layouts such as radial staggered and Fermat's spiral distribution are evaluated and different design points in a year are considered for the analysis.
