TO-A extends the lifespan of .

TO-A, T-110, and TO-A are sold as oral probiotic preparations and reportedly exhibit many beneficial effects on the health of hosts, including humans and livestock. In this study, we compared the ability of these clinically applied probiotic bacteria with OP50 in extending the lifespan of . To compare the lifespan-extending effects of the three bacteria, experiments were performed using a nematode growth medium containing a small amount of trypticase soy agar. The maximum lifespans of worms fed TO-A, T-110, or TO-A increased by 11, 12, and 26%, respectively, compared with worms fed OP50. In addition, we conducted a metabolomic analysis of methanol extracts of TO-A cells, which exhibited the strongest lifespan-extending effect on among the probiotic bacteria tested in this study. As a result, 59 candidate substances involved in extending the lifespan of were identified in TO-A cells.