Cyanobacteria are highly interesting microbes with the capacity for oxygenic photosynthesis. They fulfill an important purpose in nature but are also potent biocatalysts. This chapter gives a brief overview of this diverse phylum and shortly addresses the functions these organisms have in the natural ecosystems. Further, it introduces the main topics covered in this volume, which is dealing with the development and application of cyanobacteria as solar cell factories for the production of chemicals including potential fuels. We discuss cyanobacteria as industrial workhorses, present established chassis strains, and give an overview of the current target products. Genetic engineering strategies aiming at the photosynthetic efficiency as well as approaches to optimize carbon fluxes are summarized. Finally, main cultivation strategies are sketched.
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http://dx.doi.org/10.1007/10_2023_217 | DOI Listing |
Metab Eng
January 2025
Engineering Biology Research Center, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan; Graduate School of Science, Technology, and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe, Hyogo, 657-8501, Japan; Research Center for Sustainable Resource Science, RIKEN, 1-7-22 Suehiro, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan. Electronic address:
Polyhydroxyalkanoate (PHA) is an attractive bio-degradable plastic alternative to petrochemical plastics. Photosynthetic cyanobacteria accumulate biomass by fixing atmospheric CO, making them promising hosts for sustainable PHA production. Conventional PHA production in cyanobacteria requires prolonged cultivation under nutrient limitation to accumulate cellular PHA.
View Article and Find Full Text PDFPhotosynth Res
January 2025
Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
The Orange Carotenoid Protein (OCP) is a unique water-soluble photoactive protein that plays a critical role in regulating the balance between light harvesting and photoprotective responses in cyanobacteria. The challenge in understanding OCP´s photoactivation mechanism stems from the heterogeneity of the initial configurations of its embedded ketocarotenoid, which in the dark-adapted state can form up to two hydrogen bonds to critical amino acids in the protein's C-terminal domain, and the extremely low quantum yield of primary photoproduct formation. While a series of experiments involving point mutations within these contacts helped us to identify these challenges, they did not resolve them.
View Article and Find Full Text PDFFunct Integr Genomics
January 2025
The Energy and Resources Institute, Lodi Road, New Delhi, 110003, India.
The major limiting factor of photosynthesis in C3 plants is the enzyme, rubisco which inadequately distinguishes between carbon dioxide and oxygen. To overcome catalytic deficiencies of Rubisco, cyanobacteria utilize advanced protein microcompartments, called the carboxysomes which envelopes the enzymes, Rubisco and Carbonic Anhydrase (CA). These microcompartments facilitate the diffusion of bicarbonate ions which are converted to CO by CA, following in an increase in carbon flux near Rubisco boosting CO fixation process.
View Article and Find Full Text PDFSci Rep
December 2024
Sustainability Solutions Research Lab, Faculty of Engineering, University of Pannonia, Egyetem Str. 10, Veszprém, 8200, Hungary.
Ensuring everyone enjoys healthy lifestyles and well-being at all ages, Progress has been made in increasing access to clean water and sanitation facilities and reducing the spread of epidemics and diseases. The synthesis of nano-particles (NPs) by using microalgae is a new nanobiotechnology due to the use of the biomolecular (corona) of microalgae as a capping and reducing agent for NP creation. This investigation explores the capacity of a distinct indigenous microalgal strain to synthesize silver nano-particles (AgNPs), as well as its effectiveness against multi-drug resistant (MDR) bacteria and its ability to degrade Azo dye (Methyl Red) in wastewater.
View Article and Find Full Text PDFFront Plant Sci
November 2024
Key Laboratory of Biological Resources and Genetic Engineering of Xinjiang, College of Life Science and Technology, Xinjiang University, Urumqi, China.
Introduction: Phytoremediation is a safe and green technology for the remediation of heavy metal pollution, a global environmental problem. Bryophytes are well known for their special physiological properties, but there is little research on the use of bryophytes for phytoremediation.
Methods: In this indoor experiment, the impacts of 40 days of Cd pollution (1 (T1), 5 (T2), 10 (T3) mg·L) on Cd absorption, growth and physiological characteristics, and phyllosphere bacterial diversity of were explored.
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