Front Plant Sci
Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, China.
Published: March 2023
Introduction: Identification of stable major quantitative trait loci (QTLs) for yield-related traits is important for yield potential improvement in wheat breeding.
Methods: In the present study, we genotyped a recombinant inbred line (RIL) population using the Wheat 660K SNP array and constructed a high-density genetic map. The genetic map showed high collinearity with the wheat genome assembly. Fourteen yield-related traits were evaluated in six environments for QTL analysis.
Results And Discussion: A total of 12 environmentally stable QTLs were identified in at least three environments, explaining up to 34.7% of the phenotypic variation. Of these, for thousand kernel weight (TKW), () for plant height (PH), spike length (SL) and spikelet compactness (SCN), for PH, and for total spikelet number per spike (TSS) were detected in at least five environments. A set of Kompetitive Allele Specific PCR (KASP) markers were converted based on the above QTLs and used to genotype a diversity panel comprising of 190 wheat accessions across four growing seasons. (), and were successfully validated. Compared with previous studies, and should be novel QTLs. These results provided a solid foundation for further positional cloning and marker-assisted selection of the targeted QTLs in wheat breeding programs.
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http://dx.doi.org/10.3389/fpls.2023.1138696 | DOI Listing |
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Genomic selection is a widely used quantitative method of determining the genetic value of an individual from genomic information and phenotypic data. In this study, we used a large, multi-year training population of 3248 individuals from the University of Florida strawberry (Fragaria × ananassa Duchesne) breeding program. We coupled this training population with a test population of 1460 individuals derived from 20 biparental families.
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